The present study shows the effect of administration of LSFP for sixty days on serum estrogen level of female Wistar rats received 300, 500 and 1000 mg kg-1 body weight of the LSFP. Female Wistar rats (180–200 g) were divided into four groups. Grp I: control, Grp II: low dose (300 mg/kg), Grp III: medium dose (500 mg/kg.) Grp IV: higher dose (1000 mg/kg). Prescribed doses of LSFP suspended in gum acacia (2%) were administered orally for 60 days. On day 60th blood was withdrawn by retro-orbital puncture for estimation of serum estrogen hormone level. Control group was compared with higher dose treatment groups. Histology of vital organs was performed. Significant increase in serum Estrogen hormone levels observed at the doses 1000 mg/kg of LSFP, while no significant differences were found at 300 mg/kg and 500 mg/kg doses of LSFP when compared to control group rats. While significant increase in ovarian and uterine weights were observed at the doses 500 mg/kg and 1000 mg/kg of LSFP and no changes were observed at the doses 300 mg/kg, when compared to control group rats. Significant increase in serum estrogen hormone level and ovarian and uterine weights. The phytochemical analysis shows presence of major chemical constituents as flavonoids, phytosterols (β-sitosterol, fucosterol, campesterol), alkaloids, 5-alpha-reductase enzyme inhibitors (oleic acid, Linoleic acid, palmitic acid and steric acid). On the basis of above evidences it may possible that the presence of phytosterols, 5-alpha-reductase enzyme inhibitors may be responsible for the observed results.
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